Enzyme kinetics of multiple alternative substrates

An innovative theoretical approach that enables the complete characterisation of enzyme– substrate and enzyme–substrate–competitor reactions is generalised to systems with multiple alternative substrates. Based on the quasi-steady-state assumption, time-dependent closed form solutions are presented for cases with even, weak and mixed substrate competition. The analytic framework should facilitate the development of computational fitting procedures for progress curves, simplifying the measuring process and increasing the reliability of reaction constant estimates.